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紫斑谷螟Pyralis farinalis是常见的仓库害虫之一,它的识别和鉴定主要依靠传统的形态学鉴定方法,但对于残缺的虫体或幼虫,形态学鉴定方法则无法识别。本研究运用DNA条形码技术对送检的残缺幼虫进行鉴定。以提取到的样品基因组为模板,利用通用引物扩增其线粒体COI基因,所得基因序列与Gen Bank数据库中下载的螟蛾属5个种的14条序列进行序列比对,发现未知样品序列与紫斑谷螟的序列相似性最高,达到99.8%,遗传距离最小,为0.002-0.003。以玉米螟作为外群,采用K2P模型,以邻接法构建系统进化树,结果样品与紫斑谷螟聚为一枝。上述结果表明,残缺幼虫为紫斑谷螟的幼虫,这一结果为有效进行害虫防治提供依据。
Pyralis farinalis is one of the most common pests in the storehouse. Its identification and identification rely mainly on the traditional morphological identification methods. However, morphological identification methods can not be identified for the incomplete larvae or larvae. In this study, DNA barcodes were used to identify the missing larvae. Using the extracted genome of the sample as a template, the mitochondrial COI gene was amplified by universal primers. The sequence of the obtained gene was compared with 14 sequences of 5 species of Pyralidae downloaded from Gen Bank database. It was found that the sequence of the unknown sample and the purple spot Ceratoides have the highest sequence similarity of 99.8% with the smallest genetic distance of 0.002-0.003. With the corn borer as the outer group, the K2P model was used to construct the phylogenetic tree by the adjoining method. As a result, the sample was clustered with the purple spot moth. The above results show that the larvae larvae are larvae of the purple spot moth, the results provide the basis for effective pest control.