Objective:It is known that chronic hepatitis B virus(HBV) infection is a main risk factor for hepatocellular carcinoma(HCC).To assess the effect of HBV infection and its interaction with other factors on the risk for HCC,a hospital-based case-control study was carried out in Northeast China. Methods:A total of 384 cases with hepatocellular carcinoma and 432 controls without evidence of liver diseases were enrolled in the study.Blood samples were collected to detect the serum markers of hepatitis B virus(HBV) and hepatitis C virus(HCV) and questionnaires about lifestyle and family tumor history were performed in all subjects. Results:The total infection rate of HBV in hepatocellular carcinoma cases was 70.8%and 10.0%in non-liver disease controls.There was a statistically significant difference(P<0.0001) between cases and controls(OR= 22.0; 95%CI:15.0-32.3).Interaction analysis indicated that in HBV chronic carriers with HCV infection or alcohol consumption or family HCC history,the risk for HCC increased(OR=41.1,95%CI:20.2-83.9,OR=125.0,95%CI: 66.5-235.2;OR=56.9,95%CI:27.2-119.3 respectively).In addition,hepatitis B history,HCV infection,hepatic cirrhosis and family history of HCC were also potential HCC independent risk factors. Conclusion:We confirmed that HBV is a chief risk factor for hepatocellular carcinoma and accounts for 67.7%of all hepatocellular carcinoma in Northeast China.HCV infection,alcohol intake and family history could enhance the risk for HCC in chronic HBV carriers. Objective: It is known that chronic hepatitis B virus (HBV) infection is a main risk factor for hepatocellular carcinoma (HCC). To assess the effect of HBV infection and its interaction with other factors on the risk for HCC, a hospital-based case -control study was carried out in Northeast China. Methods: A total of 384 cases with hepatocellular carcinoma and 432 controls without evidence of liver diseases were enrolled in the study. Blood samples were collected to detect the serum markers of hepatitis B virus (HBV) Results: The total infection rate of HBV in hepatocellular carcinoma cases was 70.8% and 10.0% in non-liver disease controls. There was a significant infection rate of HBV in hepatocellular carcinoma cases Significant difference (P <0.0001) between cases and controls (OR = 22.0; 95% CI: 15.0-32.3). Interaction analysis indicated that in HBV chronic carriers with HCV infection or alcohol consumption or family HCC history, the risk for HCC increased (OR = 41.1, 95% CI: 20.2-83.9, OR = 125.0, 95% CI: 66.5-235.2; OR = 56.9, 95% CI: 27.2-119.3 respectively) infection, hepatic cirrhosis and family history of HCC were also potential HCC independent risk factors. Conclusion: We confirmed that HBV is a chief risk factor for hepatocellular carcinoma and accounts for 67.7% of all hepatocellular carcinoma in Northeast China. HCV infections, alcohol intake and family history could enhance the risk for HCC in chronic HBV carriers.