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目的探讨红花黄色素、阿魏酸在抗痛经作用中的相关作用机制。方法 70只雌性SD大鼠按体重随机分为7组:正常组、模型组、田七痛经胶囊组(田七组)、红花黄色素高剂量组(红高组)、红花黄色素低剂量组(红低组)、阿魏酸高剂量组(阿高组)及阿魏酸低剂量组(阿低组),每组10只。除正常组外,大鼠股部皮下注射己烯雌酚复制大鼠痛经模型,田七组在皮下注射己烯雌酚开始时给予田七胶囊2 g·kg-1,红高组、红低组于注射第7天分别给予红花黄色素0.02 g·kg-1·d-1、0.01 g·kg-1·d-1,阿高组、阿低组于注射第7天分别给予阿魏酸0.12 g·kg-1·d-1、0.06 g·kg-1·d-1。共注射11 d。采用酶联免疫法(ELISA)检测各组大鼠子宫组织前列腺素E2(PGE2)、前列腺素F2a(PGF2a)、血浆血栓素B2(TXB2)、6酮前列腺素F1a(6-keto-PGF1a),采用逆转录-聚合酶链反应(RT-PCR)方法检测子宫组织催产素受体(OTR)mRNA及加压素受体(VPR)mRNA的表达情况。结果与模型组比较,各药物处理组扭体反应均有所改善,但无统计学差异(P均>0.05)。与模型组比较,田七组、阿高组、阿低组PGE2含量均有不同程度升高(P<0.01或P<0.05);而田七组、阿高组、阿低组、红高组PGF2a含量及PGF2a/PGE2的比值均有不同程度降低(P<0.01或P<0.05);各药物处理组TXB2及TXB2/6-keto-PGF1a的比值均显著降低(P均<0.01)。与正常组比较,模型组大鼠子宫OTRmRNA和VPRmRNA相对表达量显著增高(P均<0.01);与模型组比较,田七组OTRmRNA、VPRmRNA相对表达量均下降(P均<0.05),阿高组OTRmRNA相对表达量下降(P<0.05);其他组组间比较无统计学差异(P均>0.05)。结论阿魏酸抗痛经作用可能与调节前列腺素系统及调节痛经大鼠子宫OTR表达有关,红花黄色素可调节前列腺素系统但对OTR及VPR表达无明显影响。
Objective To explore the related mechanism of safflower yellow and ferulic acid in anti-dysmenorrhea. Methods 70 female SD rats were randomly divided into 7 groups according to body weight: normal group, model group, Tianqi Tongjing capsule group (Tianqi group), safflower yellow high dose group (Honggao group), safflower yellow pigment low Dose group (red low group), ferulic acid high dose group (high group) and ferulic acid low dose group (low group), 10 in each group. In addition to the normal group, rats were injected subcutaneously with diethylstilbestrol for dysmenorrhea model in rats. Tianqi group was given Tianqi Capsule 2 g · kg-1 at the beginning of subcutaneous injection of diethylstilbestrol. At the seventh day after injection, Respectively, were given safflower yellow pigment 0.02 g · kg-1 · d-1, 0.01 g · kg-1 · d-1, high group, low group on the 7th day after injection of ferulic acid 0.12 g · kg- 1 · d-1,0.06 g · kg-1 · d-1. Co-injection of 11 d. The levels of prostaglandin E2 (PGE2), prostaglandin F2a (PGF2a), TXB2, 6-keto-PGF1a in rat uterine tissue were detected by enzyme linked immunosorbent assay (ELISA) Uterine tissue Oxytocin receptor (OTR) mRNA and vasopressin receptor (VPR) mRNA expression were detected by reverse transcription-polymerase chain reaction (RT-PCR) Results Compared with the model group, the writhing response of each drug treatment group was improved, but there was no significant difference (all P> 0.05). Compared with model group, the contents of PGE2 in Tianqi group, A high group and A low group increased to some extent (P <0.01 or P <0.05), while those in Tianqi group, A high group, A low group, The ratio of PGF2a and PGF2a / PGE2 was decreased to some extent (P <0.01 or P <0.05). The ratios of TXB2 and TXB2 / 6-keto-PGF1a in each drug treatment group were significantly decreased (all P <0.01). Compared with the normal group, the relative expression of OTR mRNA and VPR mRNA in the model group was significantly increased (all P <0.01). Compared with the model group, the relative expression levels of OTR mRNA and VPR mRNA in Tianqi group were decreased (P <0.05) Group OTR mRNA relative expression decreased (P <0.05); other groups showed no significant difference (P all> 0.05). Conclusion The anti-dysmenorrhoea effect of ferulic acid may be related to the regulation of prostaglandin system and the regulation of OTR expression in uterus in dysmenorrhea rats. Safflower yellow pigment may regulate the prostaglandin system but has no obvious effect on OTR and VPR expression.