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目的建立辛夷挥发油指纹图谱测定方法,为辛夷药材的质量控制提供可靠方法。方法采用水蒸气蒸馏法提取不同产地的辛夷的挥发油;用毛细管气相色谱技术测定指纹图谱。色谱条件:DB-17石英毛细管色谱柱(30 m×0.25 mm,0.25μm,美国Agilent公司);氢火焰离子化检测器;进样口温度为250℃;检测器温度为250℃;柱温以65℃为起始温度,以2℃.min-1升温至90℃,以8℃.min-1升温至145℃,以2℃.min-1升温至155℃,以10℃.min-1升温至250℃,保持5 min。载气为氮气,流速为1.0 mL.min-1,分流比为5∶1,进样量为1μL。结果建立了辛夷药材的GC指纹图谱,标定了23个共有峰。结论该方法可为更好的控制辛夷药材的内在质量提供科学依据。
OBJECTIVE To establish a method for the determination of volatile oil in Magnolia biondii, provide a reliable method for the quality control of Xinyi medicinal materials. Methods Volatile oils of Magnolia biondii from different habitats were extracted by steam distillation. The fingerprints were determined by capillary gas chromatography. Chromatographic conditions: DB-17 quartz capillary column (30 m × 0.25 mm, 0.25 μm, Agilent USA); flame ionization detector; inlet temperature was 250 ℃; detector temperature was 250 ℃; 65 ℃ as the initial temperature, the temperature was raised to 90 ℃ at 2 ℃ .min-1, the temperature was raised to 145 ℃ at 8 ℃ .min-1, the temperature was raised to 155 ℃ at 2 ℃ .min-1, Heat to 250 ° C for 5 min. The carrier gas is nitrogen, the flow rate is 1.0 mL.min-1, the split ratio is 5: 1, and the injection volume is 1 μL. Results The GC fingerprint of Xinyi was established and 23 common peaks were calibrated. Conclusion This method can provide a scientific basis for better control of the intrinsic quality of Radix.