用放射免疫分析法测定大鼠离体黄体细胞孕酮含量,观察了人工合成的12种小分子肽对大鼠黄体细胞的影响。结果表明,Gly-Tyr-NH_2和Gly-Ser-Lys 有抑制黄体细胞基础孕酮分泌作用,还抑制hCG 诱导的孕酮分泌;Lys-Tyr-NH_2,Thr-Ser-Lys,Gly-Tyr-Lys 抑制hCG 诱导的孕酮分泌;(Gly-Tyr)_2=Lys 则增强基础及hCG 诱导的孕酮分泌;其余6种小分子肽(Gly-Gly,Gly-D—Tyr,Tyr-D—Tyr,Thr-Ser-Tyr,Thr-Asn-Tyr-Thr-NH_2,Tyr-Tyr-His-Lys-Ser),对大鼠黄体细胞孕酮分泌没有影响。同时讨论了其构效关系及可能的作用机制。 The contents of progesterone in rat luteal cells were measured by radioimmunoassay. The effects of 12 small peptides synthesized on rat luteal cells were observed. The results showed that Gly-Tyr-NH2 and Gly-Ser-Lys could inhibit the progesterone secretion of luteal cells and inhibit the secretion of progesterone induced by hCG. Lys-Tyr-NH2, Thr-Ser-Lys and Gly-Tyr-Lys Gly-Gly, Tyr-D-Tyr, Tyr-D-Tyr, Gly-Gly, Tyr- Thr-Ser-Tyr, Thr-Asn-Tyr-Thr-NH_2, Tyr-Tyr-His-Lys- Ser) had no effect on the progesterone secretion of rat luteal cells. At the same time, the structure-activity relationship and its possible mechanism are discussed.