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目的:分析临床患者交叉配血试验中使用不同抗人球蛋白试剂结果不一致的因素,为临床出现抗人球蛋白法疑难配血提供借鉴及处理对策。方法:采用广谱及单特异性抗人球蛋白检测卡对患者进行血清学检测,并应用不同抗人球蛋白试剂进行试管法复查,其中交叉配血试验将患者血清56℃30min处理后再进行卡式法及试管法验证。结果:2例患者样本均为使用单特异性抗-IgG试剂配血相合,使用广谱及抗-C_3d试剂配血不合。且患者血清经56℃30min处理后使用不同试剂均配血相合。结论:结合患者临床病例资料,证实2例样本均是由血清中补体C_3d成分对使用广谱试剂的抗人球蛋白配血试验造成干扰,可给予单特异性抗-IgG试剂下配血相合血液输注,以改善其贫血症状。
OBJECTIVE: To analyze the inconsistent results of different anti-human globulin reagents used in the cross-matching blood test of clinical patients, and provide references and countermeasures for the clinical anti-human globulin method. Methods: The serological tests were performed on patients with broad-spectrum and monospecific anti-human globulin test card, and were tested by in vitro test with different anti-human globulin reagents. The cross-matching blood test was performed after the patient’s serum was treated at 56 ℃ for 30 minutes Card method and test tube verification. Results: The samples of two patients were all matched by monospecific anti-IgG reagent and matched with broad-spectrum and anti-C3d reagent. And the patient serum by 56 ℃ 30min after treatment with different reagents were matched with blood. Conclusion: According to the clinical data of patients, it is confirmed that the two samples are caused by the interference of C_3d complement of serum in the anti-human globulin with the broad-spectrum reagent, and can be administered with monospecific anti-IgG reagent Infusion to improve its anemia symptoms.