论文部分内容阅读
通过ＲＩＡ法检测脑损伤大鼠血浆及海马匀浆中ＴＮＦ－α含量变化，研究ＴＮＦ－α对海马神经元坏死的影响。结果：血浆及海马匀浆ＴＮＦ－α含量在伤后３０ｍｉｎ无明显变化，而伤后６ｈ、２４ｈ及７２ｈ均明显高于对照组，二者有明显相关性，且ＴＮＦ－α变化与病程有关。认为，脑损伤后ＴＮＦ－α增高是引起海马缺血、神经元坏死的重要因素；动态测定血浆ＴＮＦ－α水平可作为脑损伤后病情发生、发展的评价指标。
The contents of TNF-α in plasma and hippocampus of rats with brain injury were detected by RIA, and the effects of TNF-α on the neuronal necrosis of hippocampus were studied. Results: The levels of TNF-α in plasma and hippocampus homogenate had no significant change 30 min after injury, but were significantly higher at 6 h, 24 h and 72 h after injury than those in control group. It is believed that the increase of TNF-αin brain injury is an important factor of hippocampal ischemia and neuron necrosis. The dynamic determination of plasma TNF-αlevel can be used as an evaluation index for the occurrence and development of brain injury.