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甘薯品种 F32 82 的浸取液 ,经硫酸铵分级 ,甲壳素层析后 ,再经分子筛过滤 ,获得在 PAGE上呈现单一蛋白质带的甘薯凝集素 ( Ipomoea Batatas L ectin,IBL)。IBL对鸽、兔、人血等红细胞均有凝集作用 ,其中对鸽血的凝集活性最高 ,最低凝集浓度为 0 .0 5μg/ ml。IBL除凝集红细胞外 ,还可凝集小鼠脾细胞和肿瘤细胞 ;其对热相当稳定 ,90℃加热 10 min,仍有部分活性 ;IBL对酸处理较碱处理稳定 ,但可被阿拉伯糖或乳糖等解除凝集活性。IBL的中性糖含量为 5.4 %。
The extract of sweet potato variety F3282 was fractionated by ammonium sulfate, chromatographed by chitin and then filtered through molecular sieve to obtain Ipomoea Batatas Lectin (IBL) which showed a single protein band on PAGE. IBL on the pigeons, rabbits, human blood and other red blood cells have agglutination, in which the highest blood coagulation activity, the lowest agglutination concentration of 0. 05μg / ml. In addition to aggregating erythrocytes, IBL can also agglutinate mouse spleen cells and tumor cells; it is relatively stable to heat and still has some activity when heated at 90 ° C for 10 min; IBL is more stable than the alkaline treatment but can be treated with arabinose or lactose Such as lifting the agglutination activity. IBL neutral sugar content of 5.4%.