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目的:探讨S期激酶相关蛋白2(SKP2)对宫颈癌HeLa细胞增殖及相关细胞调节因子的影响。方法:利用siRNA技术,下调HeLa细胞中SKP2基因表达,流式细胞术观察干扰后肿瘤细胞凋亡情况。结果:HeLa细胞经SKP2 siRNA转染后有效降低细胞内SKP2表达,SiRNA对HeLa细胞生长有显著抑制作用,χ2=25.21,P=0.026;促使细胞凋亡增加,早期凋亡、晚期凋亡和坏死细胞分别是对照组的14.6、22.4和25倍,χ2=20.19,P=0.005;G0/G1期细胞数占76.51%,明显增加,χ2=18.11,P=0.0016;同时使p27和p-p53蛋白表达水平明显增加,伴c-myc mRNA和Cyclin A mRNA的表达水平明显降低(χ2=18.25,P=0.004),p27mR-NA表达水平显著增高,χ2=20.32,P=0.005。结论:SKP2为宫颈癌治疗的一个潜在靶点,在宫颈癌进展中的具体机制仍需进一步研究。
Objective: To investigate the effect of S-phase kinase-related protein 2 (SKP2) on the proliferation of cervical cancer HeLa cells and related cell regulators. Methods: The SKP2 gene expression in HeLa cells was down-regulated by siRNA and the apoptosis of tumor cells was observed by flow cytometry. Results: Transfection of HeLa cells with SKP2 siRNA effectively reduced the expression of SKP2 in cells. SiRNA significantly inhibited the growth of HeLa cells (χ2 = 25.21, P = 0.026), which led to the increase of apoptosis, early apoptosis, late apoptosis and necrosis Cells in control group were 14.6, 22.4 and 25 times respectively, χ2 = 20.19, P = 0.005; cells in G0 / G1 phase accounted for 76.51%, significantly increased, χ2 = 18.11, P = 0.0016; The expression of c-myc mRNA and Cyclin A mRNA was significantly decreased (χ2 = 18.25, P = 0.004), and the expression of p27mR-NA was significantly increased, χ2 = 20.32, P = 0.005. Conclusion: SKP2 is a potential therapeutic target for cervical cancer. The specific mechanism of cervical cancer progression remains to be further studied.