目前,国内胆汁胆红素含量的测定方法有乙醇法、氯仿-乙醇法,氯仿-甲醇法。本法是在氯仿-甲醇法基础上的改进。氯仿-甲醇法是利用一定比例的氯仿、甲醇混合溶媒为稀释剂,经重氮化偶联显色测定胆汁中胆红素含量的方法,实践证明,该法的精度和准确度良好,对胆红素生产工艺的评价,具有较好的科学性。然而,在操作过程中,我们发现,加入氯仿后,胆汁稀释液会形成乳浊液,这种粗分散液对测定结果有可能带来影响;而且,操作比较烦琐、费时。针对上述情况,我们对该法 At present, the determination of bilirubin content in the domestic ethanol method, chloroform - ethanol method, chloroform - methanol method. This law is based on the chloroform - methanol method based on the improvement. Chloroform - methanol method is the use of a certain percentage of chloroform, methanol mixed solvent as a diluent, diazotization coupling determination of bilirubin color method, the practice proved that the accuracy and accuracy of the method of gallbladder Evaluation of the production process of red pigment, with good scientific. However, during the operation, we found that after the addition of chloroform, the bile diluent will form an emulsion, which may have an impact on the measurement result. Moreover, the operation is cumbersome and time-consuming. In view of the above situation, we are against the law