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合成了可用作脂肪青霉素酰基转移酶活性测定的发色底物 2 -硝基 -5 [(己酰 ) -氨基 ]苯甲酸和 2 -硝基 -5 [(辛酰 ) -氨基 ]苯甲酸。在波长 40 5 nm,能检测在酶催化水解过程中所释放的发色团 2 -硝基 -5 -氨基 -苯甲酸。来自淡紫链霉菌 (Strep-tomyces lavendulae)的青霉素酰基
The chromogenic substrates 2 - nitro - 5 [(hexanoyl) - amino] benzoic acid and 2 - nitro - 5 [(octanoyl) amino] benzoic acid were synthesized for the determination of lipase penicillin acylase activity . At a wavelength of 40 5 nm, the chromophore 2 - nitro - 5 - amino - benzoic acid released during enzymatic hydrolysis can be detected. Penicillin acyl from Strep-tomyces lavendulae