[Objective] To determine the optimal enzyme extracting process of polysaccharides from Coix lachrymal-jobi L. stems. [Method] The cellulase extraction process of polysaccharides from C. lachrymal-jobi stems was optimized by means of orthogonal test design. And the content of polysaccharides in extracts was determined by spectrophotometry. [Result] The wavelength of the maximum absorption was 490nm. Glucose concentration showed a good linear relationship with absorbance at a range of 0.010 to 0.100mg/ml (R=0.9993). The optimal technological conditions for the extraction of polysaccharides were as follows: pH value was 5, amount of enzyme was 6.0ml, the reaction temperature was 40℃, and enzymatic hydrolysis time was 150min. Under these conditions, the mean extraction rate of polysaccharides was 19.62% with RSD of 0.21%. [Conclusion] The enzyme extracting procedure is reasonable, economical and feasible. [Objective] To determine the optimal enzyme extracting process of polysaccharides from Coix lachrymal-jobi L. stems. [Method] The cellulase extraction process of polysaccharides from C. lachrymal-jobi stems was optimized by means of orthogonal test design. And the content of Polysaccharide in extracts was determined by spectrophotometry. [Result] The wavelength of the maximum absorption was 490 nm. Glucose concentration showed a good linear relationship with absorbance at a range of 0.010 to 0.100 mg / ml (R = 0.9993). The optimal technological conditions for the extraction of polysaccharides were as follows: pH value was 5, amount of enzyme was 6.0 ml, the reaction temperature was 40 ° C and enzymatic hydrolysis time was 150 min. Under these conditions, the mean extraction rate of polysaccharides was 19.62% with RSD of 0.21%. [Conclusion] The enzyme extracting procedure is reasonable, economical and feasible.