背景:孤啡肽与缺血缺氧关系密切,可能在胎儿窘迫、新生儿窒息的发生发展中起到重要作用。目的:观察宫内缺血缺氧胎鼠下丘脑及外周血孤啡肽含量的变化,分析孤啡肽在围生期缺血缺氧中的作用。设计:随机对照动物实验。单位:解放军第二军医大学长海医院妇产科。材料:实验于2002-05/2003-09在第二军医大学长海医院妇产科进行,选择Wistar雌性大鼠12只,平均体质量260g。方法:将12只雌鼠随机分成3组:对照组、钳夹子宫血管10min组和钳夹子宫血管20min组,每组4只。使各组雌鼠受孕,在妊娠第21天,将各组雌鼠剖腹,对照组直接取出胎鼠,产胎鼠19只;钳夹子宫血管10min组钳夹子宫血管10min后取出胎鼠,产胎鼠21只;钳夹子宫血管20min组钳夹子宫血管20min后取出胎鼠,产胎鼠17只。均成活,所有胎鼠做Apgar评分并迅速断头,收集躯干血并取出全脑,采用放射免疫测定法测定各组胎鼠下丘脑及外周血孤啡肽的含量。主要观察指标:各组胎鼠下丘脑及外周血孤啡肽的含量。结果:57只胎鼠全部进入结果分析。①钳夹子宫血管10min组胎鼠下丘脑及外周血孤啡肽的含量分别为(71±14)pg/g及(31±7)ng/L;钳夹子宫血管20min组胎鼠下丘脑及外周血孤啡肽的含量分别为(114±21)pg/g及(58±11)ng/L;对照组胎鼠下丘脑及外周血孤啡肽含量分别为(48±9)pg/g及(19±4)ng/L;与对照组相比,钳夹子宫血管10min和20min组胎鼠下丘脑及外周血孤啡肽含量均升高(P<0.05,P<0.01);且钳夹子宫血管20min组孤啡肽含量高于钳夹子宫血管10min组(P<0.05)。②两组胎鼠Apgar评分均低于对照组(P<0.01),其中钳夹子宫血管20min组胎鼠Apgar评分低于钳夹子宫血管10min组穴P<0.05雪。结论:围生期缺血缺氧可导致下丘脑及外周血孤啡肽的含量增加。 BACKGROUND: Orphanin is closely related to ischemia and hypoxia and may play an important role in the occurrence and development of fetal distress and neonatal asphyxia. Objective: To observe the changes of orphanin in the hypothalamus and peripheral blood of intrauterine ischemia and hypoxia mice and to analyze the role of orphanin in the perinatal period of hypoxia and hypoxia. Design: Randomized controlled animal experiments. Unit: Second Military Medical University Changhai Hospital Obstetrics and Gynecology. MATERIALS: The experiment was performed at Department of Obstetrics and Gynecology, Changhai Hospital, Second Military Medical University from May 2002 to September 2003. Twelve female Wistar rats were selected and their average body weight was 260 g. Methods: Twelve female rats were randomly divided into 3 groups: control group, uterine vascular clamp 10min group and uterine vascular clamp 20min group, 4 rats in each group. The females in each group were pregnant. On the 21st day of gestation, the females were cesarean in the control group and 19 fetuses were born in the control group. The uterine vessels were dissected for 10 minutes after clamping the uterine vessels for 10 minutes. Fetal rats 21; clamp uterine vascular 20min group clamp uterine blood vessels after 20min fetus fetus, 17 fetuses. All surviving mice were subjected to Apgar scoring and rapidly decapitated. The trunk blood was collected and the whole brain was removed. The contents of orphanin in the hypothalamus and peripheral blood were measured by radioimmunoassay. MAIN OUTCOME MEASURES: The content of orphanin in the hypothalamus and peripheral blood of fetal rats in each group. Results: All 57 fetuses were included in the result analysis. (1) The levels of orphanin in the hypothalamus and peripheral blood of fetal rats were (71 ± 14) pg / g and (31 ± 7) ng / L, respectively, in the group of clamping uterine vessels for 10 minutes; The content of orphanin in peripheral blood was (114 ± 21) pg / g and (58 ± 11) ng / L, respectively. The contents of orphanin in hypothalamus and peripheral blood in control group were (48 ± 9) pg / g And (19 ± 4) ng / L, respectively. Compared with the control group, the content of orphanin in the hypothalamus and peripheral blood in the uterine vessels of 10 min and 20 min groups were significantly increased (P <0.05, P <0.01) The content of orphanin in the clamp uterine vessels 20min group was higher than that of the clamp uterine vessels 10min (P <0.05). (2) Apgar score of fetus in both groups was lower than that of control group (P <0.01), Apgar score of fetus with uterine vessels in 20min group was lower than that of uterine vessels in 10min group (P <0.05 snow). Conclusion: Perinatal hypoxia and hypoxia can lead to the increase of content of orphanin in the hypothalamus and peripheral blood.