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采用盆栽试验方法,研究了嫁接(以黑籽南瓜为砧木)对铜胁迫下黄瓜根际土壤微生物生物量、微生物种群数量和土壤酶活性的影响.结果表明:铜胁迫下黄瓜根际土壤微生物生物量碳(MBC)和微生物生物量氮(MBN)含量显著下降,基础呼吸和代谢熵显著上升,但嫁接黄瓜根际土壤MBC和MBN含量显著高于自根黄瓜,而基础呼吸和代谢熵则显著低于自根黄瓜.铜胁迫下,根际土壤放线菌和自生固氮菌的数量显著下降,真菌数量显著上升,而细菌数量变化不显著;嫁接黄瓜根系土壤细菌、放线菌、自生固氮菌的数量显著高于自根黄瓜,而真菌数量显著低于自根黄瓜.嫁接黄瓜根际土壤脲酶、磷酸酶、蔗糖酶、过氧化氢酶活性在铜胁迫下显著高于自根黄瓜.试验结果证明嫁接使铜胁迫下黄瓜根际土壤微生物环境和酶活性得到了改善和提高,从而提高了黄瓜植株对铜胁迫的抵抗力.
The pot experiment was conducted to study the effects of grafting (using black-seed pumpkin as rootstock) on soil microbial biomass, microbial population and soil enzyme activities in the rhizosphere of cucumber under copper stress. The results showed that the effects of copper stress on rhizosphere soil microbial biomass The contents of MBC and MBN decreased significantly, and the basic respiration and metabolic entropy increased significantly. However, MBC and MBN contents in rhizosphere soil of grafted cucumber were significantly higher than those of cucumber, but the basic respiration and metabolic entropy were significantly increased Was lower than that of the cucumber.The amounts of actinomyces and autotrophic azotobacter in rhizosphere soil were significantly decreased and the number of fungi increased significantly but the number of bacteria did not change significantly under copper stress.The amount of soil bacteria, actinomyces, azotobacter The number of fungi was significantly lower than that of root cucumber.The activities of urease, phosphatase, sucrase and catalase in rhizosphere soil of grafted cucumber were significantly higher than those of cucumber under copper stress It was proved that the grafting could improve and enhance the microbial environment and enzyme activity in cucumber rhizosphere soil under copper stress, and thus increased the resistance of cucumber plants to copper stress.