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目的 了解环境刺激对未成熟脑可塑性的影响及其在脑损伤后功能康复中的意义。 方法 选用雄性SD大鼠制作新生大鼠缺氧缺血性脑损伤(HIBD)模型,采用早期触摸和丰富环境刺激进行干预,观察大脑感觉运动皮层及海马CA3区突触超微结构参数的变化。结果 电镜下观察到2组大鼠两脑区均存在GRAY1型(即兴奋型)突触和穿孔突触。皮质部位干预组与非干预组比较,突触界面曲率和突触后致密物厚度(PSD)均增大,穿孔突触的百分比较高。海马部位干预组突触界面曲率和PSD厚度均较非干预组增大。结论 环境刺激对HIBD大鼠功能的促进作用可能与突触超微结构参数的改变,增强突触可塑性有关。
Objective To understand the influence of environmental stimuli on immature brain plasticity and its significance in functional rehabilitation after brain injury. Methods HIBD models of neonatal rats were made by using male SD rats. The changes of synaptic ultrastructure parameters in the sensory cortex and hippocampal CA3 area were observed by the intervention of early touch and abundant environmental stimuli. Results The GRAY1 (excitatory) synapses and perforation synapses were found in both brain regions in both groups under electron microscope. Compared with the non-intervention group, the curvature of the synaptic interface and the thickness of the synapse (PSD) increased, and the percentage of perforation synapses was higher in the cortex. The synaptic interface curvature and PSD thickness in hippocampus intervention group were higher than those in non-intervention group. Conclusion The stimulative effects of environmental stimuli on the function of HIBD rats may be related to the changes of synaptic ultrastructure parameters and the enhancement of synaptic plasticity.