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用 HPLC 法测定满山红中槲皮素含量,在 C_(18)柱上以甲醇—水—冰乙酸(50∶50∶25)为流动相,检测波长370nm,满山红中槲皮素与其它成分得到良好分离,回收率为99.2%±0.26%。本文所建立的方法可应用于满山红的质量控制。
HPLC method was used to determine the quercetin content in Manchus rubrum. The mobile phase was methanol-water-glacial acetic acid (50:50:25) on a C 18 column. The detection wavelength was 370 nm. The other ingredients were well separated and the recovery was 99.2% ± 0.26%. The method established in this paper can be applied to the quality control of Manchurian red.