进行实验性胰腺移植的研究需要可靠的糖尿病动物模型。制造狗的糖尿病模型可以利用β细胞毒剂如链脲霉素(Strep tozotocin),四氧嘧啶(Alloxan)等,也可以利用手术方法。我们按照RosyEloy提出的术式做了9例狗的全胰切除,成功地制造了糖尿病模型,现介绍如下。材料和方法 9只本地杂交狗,体重13～25公斤,不拘雌雄。按每公斤体重50毫克静脉注射Amytal做诱导麻醉,然后气管内插管做乙醚维持麻醉。 (一)手术步骤和术后护理取上腹正中切口,大网膜入路显露胰 Research in experimental pancreas transplantation requires a reliable animal model of diabetes. For the manufacture of a diabetic model of a dog, β-cytotoxic agents such as Strep tozotocin, Alloxan and the like can be used, and a surgical method can also be used. We performed a total pancreatectomy of 9 dogs according to the surgical procedure proposed by Rosy Eloy and successfully created the diabetic model as follows. Materials and Methods 9 local crossbred dogs, weighing 13 to 25 kg, regardless of sex. 50 mg per kilogram of body weight intravenous injection of Amytal induction anesthesia, and then endotracheal intubation tube to maintain anesthesia. (A) surgical procedures and postoperative care to take the median abdominal incision, omentum approach revealed pancreatic