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目的:研究5,7-二甲氧基黄酮(DMF)诱导慢性髓性白血病(CML)急变期细胞系K562细胞凋亡和调控livin基因表达作用。方法:体外培养K562细胞系细胞。碘化丙啶(PI)染色流式细胞术(FCM)分析细胞凋亡率。RT-PCR检测livin基因mRNA表达。Western blot分析livin蛋白表达。结果:DMF(5、10、20μM)增高K562细胞凋亡率,呈浓度依赖性(P<0.05)。DMF有效降低K562细胞livin mRNA和蛋白表达水平。结论:DMF诱导K562细胞凋亡作用与其下调livin基因表达相关。
AIM: To investigate the apoptosis of K562 cells induced by 5,7-dimethoxyflavone (DMF) and the expression of livin in acute myeloid leukemia (CML) cell line K562. Methods: K562 cell line was cultured in vitro. Propidium iodide (PI) staining flow cytometry (FCM) analysis of apoptosis rate. The mRNA expression of livin gene was detected by RT-PCR. Western blot analysis of livin protein expression. Results: DMF (5, 10, 20μM) increased the apoptosis rate of K562 cells in a concentration-dependent manner (P <0.05). DMF effectively reduced the expression of livin mRNA and protein in K562 cells. Conclusion: The apoptosis induced by DMF in K562 cells is related to down-regulation of livin gene expression.