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本文用生物素标记利什曼原虫动基体DNA(K-DNA)以打点杂交和Southern杂交鉴定了我国不同地区杜氏利什曼原虫(Leishmanis donovani,简称L.d.)七个分离株,并在国内首次用K—DNA杂交技术鉴定病灶组织内及白蛉体内利什曼原虫种株。结果表明,我国不同地区类型黑热病病原体间K—DNA同源性存在差异。病灶组织及白蛉体内原虫鉴定获得成功,为黑热病的诊断和流行病学研究提供了一种快速准确可行的新方法。
In this study, seven isolates of Leishmanis donovani (Ld) from different regions of China were identified by dot blot hybridization and Southern blotting using biotin-labeled Leishmania motif DNA (K-DNA) K-DNA hybridization was used to identify Leishmania species in the lesion tissue and in the whitefly. The results showed that there were differences in K-DNA homology between pathogens of different genotypes in China. The success of the identification of protozoa in the tissue of the lesion and the white flesh provides a fast, accurate and feasible new method for the diagnosis and epidemiological study of kala-azar.