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目的：本研究观察胸腺细胞凋亡小体（TAB）以评价低剂量辐射诱导的胸腺细胞凋亡的适应性反应。方法：实验用X射线全身照射Kunmin雄性小鼠，诱导剂量75mGy（D1），攻击剂量1．5或2．0Gy（D2），D1和D2间隔6h，D2照后20h检测TAB百分数。结果：D1+D2组TAB百分数明显低于D2组（P＜0．05）。此外，将75mGy照射的脾细胞外液加入2Gy照射的胸腺细胞悬液中，在培养72h明显低于2Gy照射组（P<0．05）。结论：低剂量辐射（75mGy）可改变免疫细胞的外环境，降低其凋亡，并且可诱导其后大剂量（1．5或2．Gy）照射的小鼠胸腺细胞凋亡的适应性反应。
AIM: To investigate the adaptive response of thymus apoptotic bodies (TABs) to evaluate thymocyte apoptosis induced by low dose radiation. METHODS: Kunming male mice were irradiated with X-ray to induce a dose of 75 mGy (D1) at a dose of 1.5 or 2.0 Gy (D2). The interval between D1 and D2 was 6 h, and the percentage of TAB was measured 20 h after D2 irradiation. Results: The percentage of TAB in D1 + D2 group was significantly lower than that in D2 group (P <0.05). In addition, 75mGy irradiated splenocyte extracellular fluid was added to 2Gy irradiated thymocyte suspension, which was significantly lower than 2Gy irradiated group at 72h after culture (P <0.05). CONCLUSION: Low dose radiation (75 mGy) can change the immune environment of the immune cells and reduce their apoptosis, and induce the adaptive response of the thymocytes to large doses (1.5 or 2.Gy) after irradiation.