一、经济作物组织培养(一)甘蔗台湾甘蔗组培研究显示,以甘蔗3-4mm的茎顶生长点为外植体进行培养,可从感病植株获得健康幼苗;经由愈伤组织亦可获得无病植株,但此法不易保持原品种的特性。通过生长点培养,甘蔗嵌纹病的治愈率可达95％,但成活率却仅有17.3-34％。1.组织培养在甘蔗种质保存方面的研究在悬浮细胞中添加4％:12％葡萄糖-Dim-ethylsulfoxide混合护冻剂后,经-23℃或-40℃冷冻4分钟,若以40℃迅速解冻并进行培养,仍可分化成植株。将培养细胞先用10％:5％甘油-Dimethylsulfoxide混合护冻剂在0℃处理的20-40分钟,再用G.VPlaner R201冷冻机,以每分钟降低1℃的 First, the economic crop tissue culture (A) sugarcane Taiwan Sugarcane tissue culture studies show that the top of the growing point of sugarcane stems 3-4mm as explants for the cultivation of plants can be obtained from susceptible plants; callus can also be obtained Disease-free plants, but this method is not easy to maintain the characteristics of the original breed. Through the growth point culture, the cure rate of sugarcane mosaic disease can reach 95%, but the survival rate is only 17.3-34%. 1. Tissue Culture Studies on Sugarcane Germplasm Storage After adding 4%: 12% Dim-ethylsulfoxide mixed cryogenic agent into suspension cells, the cells were frozen at -23 ℃ or -40 ℃ for 4 minutes, Thawed and cultured, can still differentiate into plants. The cultured cells were first treated with a 10%: 5% glycerol-Dimethylsulfoxide mixed cryogen at 0 ° C for 20-40 minutes and then rehydrated with a G.VPlaner R201 freezer at 1 ° C per minute