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目的 了解肝癌中细胞周期及生长调控因子的表达概况 ,探寻其在肝癌与正常肝组织中的表达差异。方法 以 2 4例肝癌及癌旁正常肝组织的总RNA反转录合成含有α 3 2 PdATP的cDNA为探针 ,与Atlas微阵列表达分析膜进行差异杂交 ,并应用半定量逆转录 聚合酶链式反应(RT PCR)及印迹法 (Northern)杂交验证。结果 在所分析的 5 88种已知基因中 ,与细胞周期及生长调控相关的基因共有 79个 ,其中TFDP 2、E2F 3、ERK等 7个在肝癌组织中表达上调 ,MAPKK和CDK3表达下调。RT PCR结果和Northern杂交的结果均证实了Atlas微阵列差异杂交的准确性。结论 通过Atlas微阵列较全面系统地研究肝癌的基因表达改变 ,这些基因的表达改变组成了一个肝癌特异的基因表达谱。一些与细胞周期及生长调控有关的差异表达基因为研究肝癌的发病机制及肝癌的发生、发展提供了有益的线索
Objective To understand the expression profile of cell cycle and growth regulators in hepatocellular carcinoma (HCC) and explore their differences in liver cancer and normal liver tissues. METHODS: cDNAs containing α 3 2 PdATP were synthesized by reverse transcription of total RNA from 24 hepatocellular carcinomas and normal liver tissues adjacent to tumors as probes. Differential hybridization with Atlas microarray expression analysis membranes was performed. Semi-quantitative RT-PCR was used. RT PCR and Northern hybridization verification. Results There were 79 genes related to cell cycle and growth regulation among the 5 908 known genes. Among them, 7 genes such as TFDP 2, E2F 3 and ERK were up-regulated in hepatocellular carcinoma, while those of MAPKK and CDK3 were down-regulated. Both RT PCR results and Northern hybridizations confirmed the accuracy of differential hybridization in Atlas microarrays. Conclusions Atlas microarrays were used to study the gene expression changes of liver cancer more systematically. The expression changes of these genes constitute a liver cancer-specific gene expression profile. Some differentially expressed genes related to cell cycle and growth regulation provide useful clues for studying the pathogenesis of liver cancer and the occurrence and development of liver cancer.