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目的:在灵芝转录组基础上,对灵芝蛋白质精氨酸甲基转移酶(PRMTs)GLPRMT1,GLPRMT2和GLPRMT3基因进行全面的生物信息学分析。方法:利用生物信息学方法对灵芝GLPRMT1,GLPRMT2和GLPRMT3基因编码氨基酸序列的理化特性、亲/疏水性、功能域、二级结构、三级结构和系统发育进化等进行分析和预测;利用转录组FPKM分析灵芝不同生长时期GLPRMT1,GLPRMT2和GLPRMT3基因的相对表达量。结果:GLPRMT1和GLPRMT2均具有完整的开放阅读框且为全长,而GLPRMT3不为全长互补脱氧核糖核酸;GLPRMT1,GLPRMT2和GLPRMT3都具有蛋白质精氨酸甲基转移酶最保守的Ado Met-MTases结构域;GLPRMT1,GLPRMT2,GLPRMT3分别与真菌的PRMT3家族,PRMT1家族和PRMT5家族聚为一支;GLPRMT2的表达量明显高于GLPRMT1和GLPRMT3,且3个蛋白质精氨酸甲基转移酶基因表达随着灵芝个体的发育均呈上升趋势。结论:本研究结果为揭示灵芝的表观调控机制提供理论基础。
OBJECTIVE: To conduct a comprehensive bioinformatic analysis of the GLPRMT1, GLPRMT2 and GLPRMT3 genes of the rhizome dexamethasone protein arginine methyltransferase (PRMTs) based on Ganoderma lucidum transcriptome. Methods: Bioinformatics methods were used to analyze and predict the physicochemical properties, pro / hydrophobicity, functional domain, secondary structure, tertiary structure and phylogenetic evolution of the coding amino acid sequences of GLPRMT1, GLPRMT2 and GLPRMT3 genes. FPKM analysis of different growth stages of Ganoderma lucidum GLPRMT1, GLPRMT2 and GLPRMT3 relative gene expression. RESULTS: Both GLPRMT1 and GLPRMT2 had a full open reading frame and were full-length, while GLPRMT3 was not full-length complementary DNA. Both GLPRMT1, GLPRMT2 and GLPRMT3 had the most conserved protein of arginine methyltransferase, Ado Met-MTases GLPRMT1, GLPRMT2 and GLPRMT3 were clustered together with PRMT3 family, PRMT1 family and PRMT5 family respectively. The expression of GLPRMT2 was significantly higher than that of GLPRMT1 and GLPRMT3, and the expression of three protein arginine methyltransferase The development of Ganoderma lucidum individuals are on the rise. Conclusion: The results of this study provide a theoretical basis for revealing the apparent regulatory mechanism of Ganoderma lucidum.