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为了解循环水鳗鲡养殖过程中调节MBBR生物滤池的工艺后微生物群落结构与代谢功能变化情况,进而寻找能否在节能的工艺下,生物滤池中的滤料微生物仍有良好的硝化作用,通过改变生物滤池依次至O2、A/O和O/A工艺,运用Biolog-ECO法分析各工艺稳定条件下生物滤池中微生物群落的代谢特征。结果显示:AWCD值总体变化趋势上O2工艺二段明显最优,其次是A/O工艺一段,而O/A工艺一段的滤料微生物对碳源利用能力相对最差;在对六类碳源的利用情况上,不同工艺对氨基酸类和胺类代谢偏好明显,其中O2工艺二段和A/O工艺一段的滤料微生物对六类碳源利用程度相对较高;O/A工艺一段在多样性指数上处于最高水平,其次是O2工艺二段和O/A工艺二段,O2工艺一段在各多样性指数上均处于较低水平;主成分分析显示,不同工艺不同生物滤池段的滤料微生物代谢基质主成分1的贡献率为32.89%,主成分2为25.91%,主成分1相关系数0.5以上的碳源有16种,主成分2有14种;在滤料微生物对31种碳源的利用上,利用明显偏好的(>4%)有氨基酸类的L-天门冬酰胺和L-精氨酸、碳水化合物中的N-乙酰-D-葡萄糖胺、多聚物中的吐温40和吐温80、酚酸类的4-羟基苯甲酸和胺类的苯乙胺,利用率较低的为羧酸类的γ-羟基丁酸、碳水化合物的D,L-α-磷酸甘油和酚酸类的2-羟基苯甲酸。实验通过调节曝气情况改变生物滤池的工艺,研究生物滤池中的微生物群落碳代谢特征,为循环水养殖系统的进一步研究提供参考依据。
In order to understand the changes of microbial community structure and metabolic function after regulating the process of MBBR biofilter in the process of rearing Eel angustifolia, we can find out whether the biofilter microorganisms still have good nitrification under the energy-saving process, By changing the biofilters to O2, A / O and O / A processes in turn, Biolog-ECO method was used to analyze the metabolic characteristics of microbial communities in biofilters under various process conditions. The results showed that: the overall trend of AWCD value was obviously superior to the second stage of O2 process, followed by the first stage of A / O process, while the first stage of O / A process had the worst utilization ability of carbon source; , The preference of different processes for amino acids and amines metabolism is obvious. Among them, the second stage of O2 process and the first stage of A / O process use relatively high degree of utilization of six types of carbon sources; the first stage of O / Sex index is at the highest level, followed by the O2 process and the O / A process of the second paragraph, O2 process in the diversity index were at a low level; principal component analysis showed that different processes of different biofilter filter The contribution rate of main component 1 of microbial metabolic substrate was 32.89%, that of main component 2 was 25.91%, that of main component 1 whose correlation coefficient was 0.5 or more was 16 and that of main component 2 was 14; On the utilization of sources, L-asparagine and L-arginine with amino acids, N-acetyl-D-glucosamine in carbohydrates, Tweens in polymers 40 and Tween 80, Phenolic acids 4-hydroxybenzoic acid and amine phenethylamine, the lower utilization of carboxylic acid 2-hydroxy-benzoic acid γ- hydroxybutyric acid, carbohydrate D, L-α- glycerophosphate and Phenolic. In the experiment, the biofilter process was changed by adjusting the aeration condition to study the carbon metabolism characteristics of microbial communities in the biofilter, which could provide a reference for further research on the recirculating aquaculture system.