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背景:急性脑损害条件下,心脏病变发生率高,影响其预后。血浆单胺类递质的大量增加,可能是脑卒中后心脏产生应变效应的主要原因之一。目的:研究单胺类递质在大鼠脑出血心脏损伤中的作用,探讨脑心综合征发生、发展的机制。设计:以实验动物为研究对象,随机对照实验研究。单位:一所军医大学医院的野战外科研究所神经内科病房。材料:实验在大坪医院神经内科实验室完成。动物选用健康Wistar大鼠70只(雌雄不拘,体质量250～300g),动物由第三军医大学大坪医院动物中心提供。干预:大鼠右侧尾状核注射含胶原酶(1u/μL)和肝素(7u/μL)的生理盐水1μL以建立脑出血模型;对照组改注射生理盐水1μL。主要观察指标:采用高效液相-电化学法测定血浆去甲肾上腺素(noradrenaline,NE)含量,采用酶反应速率法测定血清肌酸磷酸激酶同工酶MB型(creatinekinase-MB,CK-MB)的变化。结果:大鼠脑内血肿开始形成的6h血浆去甲肾上腺素(noradrenaline,NE)、血清肌酸磷酸肌酶同工酶MB型(creatinekinase-MB,CK-MB)均显著升高犤分别为(2.782±0.244)μg/L和(3.504±0.742)μkat/L犦,并以脑出血24h血肿高峰期时最为显著犤分别为(3.517±0.200)μg/L,(5.474±1.540)μkat/L犦,随后逐渐下降,72h血浆NE降至术前水平犤(1.663±0.094)μg/L犦,而血清
Background: Under the condition of acute brain damage, the incidence of heart disease is high, which affects its prognosis. A large increase in plasma monoamine neurotransmitters may be one of the major causes of the strain-induced cardiac effects after stroke. OBJECTIVE: To study the role of monoamine neurotransmitters in the injury of rat brain with intracerebral hemorrhage and to explore the mechanism of brain-heart syndrome. Design: The experimental animals as the research object, randomized controlled experimental study. Unit: A Military Medical University Hospital Field Surgery Institute neurology ward. Materials: The experiment was performed in Daping Hospital Neurology Laboratory. 70 healthy Wistar rats (male and female, body weight 250-300g) were selected as animals and animals were provided by Animal Center of Daping Hospital, Third Military Medical University. Intervention: 1μL of saline containing collagenase (1u / μL) and heparin (7u / μL) was injected into the right caudate nucleus of rat to establish intracerebral hemorrhage model. The control group was injected with 1μL of normal saline. MAIN OUTCOME MEASURES: The content of plasma norepinephrine (NE) was determined by high performance liquid chromatography-electrochemical method. The levels of serum creatine kinase-MB (CK-MB) The change. Results: The levels of noredrenaline (NE) and creatine kinase-MB (CK-MB) in 6h plasma formed in rat intracerebral hemorrhage were significantly increased (2.782 ± 0.244 μg / L and 3.504 ± 0.742 μkat / L 犦, respectively. The most significant hemorrhagic peak was (3.517 ± 0.200) μg / L and (5.474 ± 1.540) μkat / L 以, Then gradually decreased, 72h plasma NE decreased to preoperative level 犤 (1.663 ± 0.094) μg / L 犦, and serum