环磷酰胺在贮藏过程中,经常产生变质问题。若采用常规的检验法——定氯定磷法,难以反映出其内在质量,为此尝试改用红外光谱法。为克服直接测定层厚的困难,采用加入一定量的内标后测定,分别测得9.5μm与4.9μm处吸收值,并根据二者吸收比值与浓度绘制标准曲线,供试品可根据它的吸收比值求得其浓度。 Cyclophosphamide in the storage process, often produce deterioration problems. If the conventional test method - set chlorophosphate method, it is difficult to reflect the inherent quality, to try to use infrared spectroscopy. To overcome the difficulty of direct determination of layer thickness, adding a certain amount of internal standard determination, were measured 9.5μm and 4.9μm absorption value, and according to the absorption ratio and concentration of the two draw a standard curve for the test sample according to its Absorption ratio obtained its concentration.