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用免疫组织化学和组织化学方法对大鼠三叉神经脊束核尾侧亚核（Ｖｃ）内ＳＰ受体阳性神经元、ＦＯＳ阳性神经元及初级传入Ｃ纤维和终末的分布及相互之间的关系进行了观察。ＳＰ受体阳性神经元的胞体及树突主要分布在此核的Ⅰ、Ⅱ层，Ⅰ层多于Ⅱ层；１０％福尔马林刺激大鼠口周区后诱导的ＦＯＳ阳性神经元分布于此核的Ⅰ～Ⅳ层及其内侧的网状结构，但主要集中在Ⅰ、Ⅱ层，Ⅱ层多于Ⅰ层；ＢＳＩ－Ｂ４标记的初级传入Ｃ纤维及其终末也密集地分布于此核的Ⅰ、Ⅱ层，且Ⅱ层多于Ⅰ层。多重反应的结果显示：在Ｖｃ的Ⅱ层和Ⅰ层内，ＳＰ受体阳性神经元的胞体及树突和ＦＯＳ阳性神经元胞体的周围可见许多ＢＳＩ－Ｂ４标记的纤维及终末；部分ＳＰ受体阳性神经元也呈ＦＯＳ阳性，少数ＳＰ受体和ＦＯＳ的双重阳性神经元周围也有ＢＳＩ－Ｂ４标记的纤维和终末。以上结果说明：（１）传递面口部痛信号的Ｃ纤维主要终止在尾侧亚核的Ⅰ、Ⅱ层，与ＳＰ受体阳性神经元的分布区重叠，提示这些Ｃ纤维可能含ＳＰ；（２）参与西口部痛信息传递的ＦＯＳ阳性神经元主要分布于Ｖｃ的Ⅱ层，它们与初级传入Ｃ纤维终末的分布区重叠，其中的部分ＰＯＳ阳性神经元也呈ＳＰ受体阳性，从而进一步地证明了ＢＳＩ－Ｂ４标记的初级传入Ｃ纤?
Immunohistochemistry and histochemical methods were used to detect the distribution of SP receptor positive neurons, FOS positive neurons and primary afferent C fibers and terminals in the subnucleus (Vc) of the rat trigeminal nucleus and between them The relationship was observed. SP receptor positive neurons cell body and dendrites are mainly distributed in the nucleus Ⅰ, Ⅱ layer, Ⅰ layer more than Ⅱ layer; 10% formalin-induced rat perioral area after FOS-positive neurons were distributed in The nucleus Ⅰ ~ Ⅳ and its inner reticular structure, but mainly concentrated in Ⅰ, Ⅱ layer, Ⅱ layer more than Ⅰ layer; BSI-B4 labeled primary afferent C fiber and its terminal are also densely distributed in This core Ⅰ, Ⅱ layer, and more than Ⅰ Ⅰ layer. The results of multiplex reaction showed that many BSI-B4-labeled fibers and terminals could be seen around the cell body and dendrites of SP receptor positive neurons and in the body of FOS positive neurons in layer II and layer I of Vc. Some SP receptors Body-positive neurons are also FOS-positive, a small number of SP receptors and FOS dual positive neurons also have BSI-B4-labeled fibers and terminals. The above results indicate that: (1) C fiber, which transmits pain signal of face and mouth, mainly terminates in layers Ⅰ and Ⅱ of the caudal subnucleus and overlaps with the distribution of SP receptor positive neurons, suggesting that these C fibers may contain SP; 2) The FOS-positive neurons participating in the pain information transmission in the western part of the mouth are mainly distributed in the second layer of Vc, which overlap with the distribution of primary afferent C-fiber terminals. Some POS-positive neurons are also SP receptor positive Further evidence of BSI-B4 labeled primary afferent C fibers?