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为探讨急性出血坏死性胰腺炎（ＡＨＮＰ）大鼠血清一氧化氮（ＮＯ）的动态变化及硝普钠的治疗作用。将１２０只Ｗｉｓｔａｒ大鼠随机分为３组，每组各４０只。ＡＨＮＰ组动物模型采用经胰胆管逆行注射５％牛磺胆酸钠建立；治疗组为在ＡＨＮＰ模型制备后经腹腔注射硝普钠（０．０５ｍｇ／１００ｇ），３小时后再重复注射１次；对照组为大鼠开腹经胰胆管逆行注射生理盐水０．５ｍｌ后关腹。动态观察各组指标的变化。结果显示：ＡＨＮＰ组大鼠血清ＮＯ和超氧化物歧化酶（ＳＯＤ）含量明显低于治疗组（Ｐ＜０．０５）；血清淀粉酶和胰腺系数则明显高于治疗组（Ｐ分别＜０．０１和＜０．０５）；此外，ＡＨＮＰ组２４小时病死率明显高于治疗组，存活时间明显短于治疗组（Ｐ＜０．０１）；ＡＨＮＰ组胰腺的病理改变也明显重于治疗组。由此表明：ＮＯ在急性出血坏死性胰腺炎的发病过程中起了重要作用，硝普钠对大鼠ＡＨＮＰ有治疗作用
To investigate the dynamic changes of serum nitric oxide (NO) in rats with acute hemorrhagic necrotizing pancreatitis (AHNP) and the therapeutic effects of sodium nitroprusside. 120 Wistar rats were randomly divided into 3 groups of 40 each. AHNP group was established by retrograde cholangiopancreatic injection of 5% sodium taurocholate. AHNP model rats were injected intraperitoneally with sodium nitroprusside (0.05mg / 100g) and injected once more after 3 hours. In the control group, the rats were anaesthetized by injecting 0.5 ml normal saline through the pancreaticobiliary duct. Dynamic observation of changes in each index. The results showed that the content of serum NO and superoxide dismutase (SOD) in AHNP group was significantly lower than that in the treatment group (P <0.05), and the serum amylase and pancreatic coefficient were significantly higher than those in the treatment group (P <0. 01 and <0.05). In addition, the 24-hour mortality rate in AHNP group was significantly higher than that in the treatment group, and the survival time was significantly shorter in the AHNP group than in the treatment group (P <0.01). The pathological changes of pancreas in AHNP group were also significantly higher than those in the treatment group. This shows that: NO in the pathogenesis of acute hemorrhagic necrotizing pancreatitis played an important role, sodium nitroprusside has a therapeutic effect on rat AHNP