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婴幼儿毛细血管瘤(ICH)常在出生后不久发生,生长迅速,数年后常自然消退。其自然消退的原因尚不清楚。为阐明ICH自然消退的原因,作者研究了7例ICH的凋亡活性及增殖活性,并与5例叶状毛细血管瘤(LCH,又称化脓性肉芽肿)进行了对比研究。后者在病理形态上与ICH相似,但不发生自然消退。 材料和方法 采用福尔马林固定的石蜡包埋块。研究方法为:①光镜检测细胞凋亡(Apop Tag染色)。②免疫组化染色:检测Ki-67,P53蛋白,bcl-2蛋白及Le~y抗原的表达。③凋亡指数及增殖指数:对凋亡的检测采用两种方法。a.检测50个高倍视野(HPF,×400)
Infantile capillary hemangioma (ICH) often occurs shortly after birth, rapid growth, often after a few years often subsided. The reason for its natural regression is not clear. To elucidate the causes of ICH spontaneous regression, the authors studied the apoptotic and proliferative activity of seven ICH patients and compared them with five cases of leafy capillary hemangioma (LCH). The latter is similar in pathological appearance to ICH but does not spontaneously regress. Materials and Methods Formalin fixed paraffin blocks were used. Research methods: ① light microscopy apoptosis (Apop Tag staining). ② Immunohistochemical staining: The expressions of Ki-67, P53 protein, bcl-2 protein and Le y antigen were detected. ③ Apoptosis index and proliferation index: The detection of apoptosis using two methods. a. Detection of 50 high power fields (HPF, × 400)