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以寒地粳稻东农428、龙稻7和松粳10为试验材料,研究分蘖期冷水胁迫下不同施氮量处理对功能叶片氮代谢关键酶活性、籽粒蛋白质含量以及产量的影响,并探讨功能叶片氮代谢关键酶活性与籽粒蛋白质含量以及产量的关系。研究结果表明,分蘖期冷水胁迫使NR,GS活性及籽粒蛋白质含量升高,GOGAT和GDH活性下降。分蘖期冷水胁迫下,增加施氮量使各品种功能叶酶活性及籽粒蛋白质含量均缓慢升高,其中NR、GS和GOGAT活性在N150处理下最高,GDH活性和籽粒蛋白质含量在N175处理下最高,可知,高施氮量不利于寒地粳稻抗冷。不同品种在冷水胁迫下酶活性表现不一,其中耐冷型品种东农428在各处理下氮代谢关键酶保持较高生理活性,龙稻7次之,松粳10最弱。分蘖期冷水胁迫下,纯氮施用量为100 kg/hm2,其产量表现最佳,3个品种表现一致。
The effects of different nitrogen application rates on the key enzyme activities of nitrogen metabolism, grain protein content and grain yield in functional leaves under cold-water stress at tillering stage were studied using cold paddy Dongnong 428, Longdao 7 and Songjing 10 as experimental materials. Relationship between key enzyme activity of nitrogen metabolism and grain protein content and yield in leaves. The results showed that cold water stress at tillering stage increased the activities of NR and GS and the contents of grain protein, but decreased the activities of GOGAT and GDH. Under the condition of cold water stress at tillering stage, increasing the amount of nitrogen fertilizer increased the functional leaf enzyme activities and grain protein contents of all cultivars, and the activities of NR, GS and GOGAT were the highest under the treatment of N150. The activity of GDH and the content of grain protein were the highest , We can see that high nitrogen application is not conducive to cold japonica rice cold. The activities of enzymes in different cultivars under different cold water stress varied. Among them, Dongnong 428, a tolerant cold-tolerant cultivar, maintained high physiological activity of key nitrogen metabolism enzymes, while Longjing 7 and Songjing 10 were the weakest. Under the condition of cold water stress at the tillering stage, the application of pure nitrogen was 100 kg / hm2, and its yield was the best. The three cultivars showed the same performance.