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目的观察益智治呆方对β-淀粉样蛋白(Aβ)诱导的大鼠脑组织星形胶质细胞蛋白(GFAP)表达及对乙酰胆碱酯酶、过氧化氢酶活性的影响,分析益智治呆方治疗老年痴呆症患者的具体作用途径。方法选取30只大鼠,平均分成对照组(脑内注射5μl生理盐水)、模型组(脑内注射Aβ1-42,10μg/5μl)以及AD模型联合益智治呆方组(治疗组,脑内注射Aβ1-42,10μg/5μl)。建模成功后治疗组采用益智治呆方0.5 mg/(kg·d)灌胃,对照组和模型组采用等量生理盐水灌胃。持续喂养之后取脑组织分析病理改变状况,使用免疫染色法检测Aβ标记部位与神经细胞毒性,应用Western blot进行GFAP检测。分析三组大鼠行为学结果。结果治疗组大鼠以及模型组大鼠脑组织切片当中的海马中央还有皮质中线旁注射区,存在大小不同的Aβ1-42阳性颗粒;神经细胞标记抗体染色之后,在模型组大鼠以及治疗组大鼠注射使用Aβ的脑组织海马区以及皮质区能够发现神经细胞的大小不够均匀,同时少数神经细胞出现变形,细胞带明显出现损伤,细胞数量显著下降,治疗组大鼠的病变程度与模型组对比要轻,同时对照组大鼠无上述变化(P<0.05)。治疗组大鼠在使用益智治呆方进行治疗之后,显著降低了神经元的损伤程度,同时降低GFAP表达水平。造模之后模型组大鼠的反应显著下降,电击次数显著上升(P<0 05),治疗组大鼠逃避反应更加敏感,电击次数显著下降(P<0 05)。结论通过应用益智治呆方,能够有效降低Aβ模型鼠GFAP表达水平,保护神经细胞,减轻脑组织损伤程度。
Objective To observe the effects of Yizhi Zhidu Decoction on β-amyloid (Aβ) -induced GFAP expression and acetylcholinesterase (AChE) activity and catalase (CAT) activity in rat brain, Cure side of the treatment of patients with Alzheimer’s specific ways of action. Methods Thirty rats were randomly divided into control group (intraperitoneal injection of 5μl normal saline), model group (intracerebral injection of Aβ1-42, 10μg / 5μl) and AD model combined with Yizhi Dudian prescription group Aβ 1-42, 10 μg / 5 μl). After the model was established, the treatment group was given intragastric administration of 0.5 mg / (kg · d) of Yizhi Decoction, while the control group and model group were given intragastric administration of normal saline. After continuous feeding, the brain tissue was taken for pathological changes. Immunofluorescence staining was used to detect Aβ-labeled sites and neurotoxicity. Western blot was used to detect GFAP. Behavioral results of three groups were analyzed. Results The Aβ1-42 positive particles in the central hippocampus and the midline of the cortex in the brain tissue sections of rats in the treatment group and in the model group were present. The Aβ1-42 positive particles of different sizes existed in the treated group and the model group. In the hippocampus and cortex regions of brain tissue of rats injected with Aβ, the size of nerve cells can be found not to be uniform, and a small number of neurons deform, the cell bands are obviously damaged and the number of cells is significantly decreased. The degree of lesion in the treatment group is similar to that of model group The contrast was lighter, while the control group rats without the above changes (P <0.05). After treatment with Yizhi Zhigui, the treatment group rats significantly reduced the degree of neuronal damage and decreased the expression of GFAP at the same time. After modeling, the response of rats in the model group decreased significantly, the number of shocks increased significantly (P <0.05), the evacuation response of the rats in the treatment group was more sensitive and the number of shocks decreased significantly (P <0.05). Conclusion Using Yizhi Zhifu Recipe can effectively reduce the expression of GFAP in Aβ model rats, protect the nerve cells and lessen the damage of brain tissue.