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Objective:To ascertain the variations of mitochondrion DNA(mtDNA) in mouse tumors and to inquire into the relationship between mutations of mtDNA and carcinogenesis.Methods:The variations of D-loop,ND3 and tRNA~(Met+Glu+Ile) gene fragments of mtDNA from six tumor cell lines of mice were analyzed by PCR technology with restriction fragment length polymorphism analysis(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP) and single strand conformation polymorphism analysis(SSCP-PCR) method.Results:ND3 and tRNA~(Met+Glu+Ile) gene fragments of mtDNA from the tumors showed no variation in 27 endonuclease sites;D-loop of mtDNA from Hca-F had an additional endonuclease sites of HinfⅠin contrast to that of the inbred mouse.Deeply analyzed by PCR-SSCP,the D-loop of mtDNA was found to possess mutations in 4 of 6 tumors.Conclusion:D-loop is the hot spot of tumor mtDNA mutation which can act as contributors to the carcinogenic process.
Objective: To ascertain the variations of mitochondrion DNA (mtDNA) in mouse tumors and to in inquire into the relationship between mutations of mtDNA and carcinogenesis. Methods: The variations of D-loop, ND3 and tRNA ~ (Met + Glu + Ile) gene fragments of mtDNA from six tumor cell lines of mice were analyzed by PCR technology with restriction fragment length polymorphism analysis (PCR-RFLP) and single strand conformation polymorphism analysis (SSCP-PCR) method. Results: ND3 and tRNA ~ (Met + Glu + Ile) gene fragments of mtDNA from the tumors showed no variation in 27 endonuclease sites; D-loop of mtDNA from Hca-F had an additional endonuclease sites of HinfIin contrast to that of the inbred mouse. Deeply analyzed by PCR-SSCP, the D-loop of mtDNA was found to possess mutations in 4 of 6 tumors. Confc: D-loop is the hot spot of tumor mtDNA mutation which can act as contributors to the carcinogenic process.