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目的：建立复方丹参片中三七皂苷R1、人参皂苷Rb1及人参皂苷Rg1含量测定方法。方法：采用HPLC法，用Diamonsil C18（250 mm×4.6 mm，5μm）色谱柱，乙腈-0.05%磷酸溶液为流动相。检测波长为203 nm，流速为1.0 mL/min，进样量为10μL。结果：三七皂苷R1线性回归方程Y=187.0X+16.16，r=0.9997，三七皂苷R1含量在0.366-3.206μg之间为良好线性关系。人参皂苷Rb1含量在0.911-9.241μg之间为良好线性关系。人参皂苷Rg1线性回归方程Y=403.6X+9.113，r=0.9994，人参皂苷Rg1含量在0.841-8.431μg之间为良好线性关系。结论：该法可以用于测定复方丹参片中三七皂苷R1、人参皂苷Rb1及人参皂苷Rg1含量，可以为药物的质量控制提供参考。“,” Objective: To establish the method to determine the content of Notoginsenoside R 1, Ginsenoside Rb1 and Gin-senoside Rg1 in Compound Danshen Tablets by HPLC. Methods: The HPLC method was applied and Diamonsil C 18(250 mm×4.6 mm, 5μm) was used; the ACN-H3PO4 (0.05%) was used as mobile phase; detective wave was 203 nm; flow rate was 10 mL/min. Results:The regression equations of notoginsenoside R1 was Y=187.0X+16.16, r=0.9997, and the linear range of notoginseno-side R1 was 0.366-3.206μg; the linear range of ginsenoside Rb1 was 0.911-9.241μg; the regression equations of ginsenoside Rg1, was Y=403.6X+9.113, r=0.9994,and the linear range of ginsenoside Rg1 was0.841-8.431 μg. Conclusion: The HPLC is promise method to determine the content of Notoginsenoside R1, Ginsenoside Rb1 and Ginsenoside Rg1, which is benefit to con-trol the quality of Compound Danshen Tablets.