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目的:探索p38信号通路在上颌突间充质细胞体外成骨分化中的调控作用。方法:取第1代E12.5 d的小鼠上颌突间充质细胞进行成骨诱导培养1周,实验组加入SB203580(p38磷酸化抑制剂)。通过免疫荧光检测磷酸化p38的表达,通过Brdu标记和免疫荧光检测细胞的增殖能力,通过ALP染色和定量PCR检测成骨标志物的表达。采用SPSS18.0软件包对数据进行统计学分析。结果 :成骨诱导可促进上颌突间充质细胞中p38的磷酸化(p-p38)。抑制p38的磷酸化,可抑制上颌突间充质细胞增殖,降低成骨标志物ALP、Runx2、OCN和OPN的表达,使ALP染色减弱。结论:p38信号通路参与调控体外培养的上颌突间充质细胞的成骨分化。
Objective: To explore the regulation of p38 signaling pathway in osteogenic differentiation of maxillary mesenchymal cells in vitro. Methods: The first generation of E12.5 d mouse maxillary mesenchymal cells were cultured for 1 week, SB203580 (p38 phosphorylation inhibitor) was added to the experimental group. The expression of phosphorylated p38 was detected by immunofluorescence, the proliferation of cells was detected by Brdu labeling and immunofluorescence, and the expression of osteogenic markers was detected by ALP staining and quantitative PCR. SPSS18.0 software package for statistical analysis of the data. RESULTS: Osteoinduction promoted phosphorylation of p38 in maxillary mesenchymal cells (p-p38). Inhibition of p38 phosphorylation can inhibit the proliferation of maxillary protrusion mesenchymal cells, reduce the expression of osteoblast markers ALP, Runx2, OCN and OPN, so that ALP staining weakened. Conclusion: The p38 signaling pathway is involved in the regulation of osteogenic differentiation of maxillary mesenchymal cells cultured in vitro.