本次试验确定了烟草中铁氧化还原蛋白酶(Fd)的初级结构,并与茄科的其他植物中该酶的结构进行了比较。 试验材料与方法:曾报道从烟草中分离铁氧化还原蛋白酶(Fd)的方法,本次只稍做改动,即用Q Sepharose FF柱、Sephacryl S-100 HR柱分别代替DEAE-纤维素柱和Sephadex G-75柱进行分离。并且在过Sephacryl S-100 HR柱之前先用饱和硫酸铵盐析,可使Fd与其他杂质分离。氨基酸序列的测定:采用气相蛋白测序仪,在以前的研究中已有报道。种属关系 This experiment determined the primary structure of ferredoxin (Fd) in tobacco and compared it with the structure of this enzyme in other plants of Solanaceae. Test materials and methods: The method of separating ferrooxidation-reducing protease (Fd) from tobacco has been reported. This time, only a slight modification was made, ie, the DEAE-cellulose column and Sephadex were replaced with Q Sepharose FF column and Sephacryl S-100 HR column, respectively. G-75 column separation. And before the Sephacryl S-100 HR column was saturated with ammonium sulfate, Fd can be separated from other impurities. Amino acid sequence determination: Gas-phase protein sequencer has been reported in previous studies. Species relationship