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目的 研究IFN γ、TNF α、IL 1和地塞米松对人原代培养肝细胞细胞间黏附分子 1(ICAM 1)表达的影响。方法 用IFN γ、TNF α和IL 1在体外诱导人原代培养肝细胞表达ICAM 1,并用细胞ELISA检测其ICAM 1表达水平 ;在用细胞因子刺激前先加入地塞米松 ,观察地塞米松对人肝细胞ICAM 1表达的影响。结果 未经刺激的人原代培养肝细胞ICAM 1表达水平很低 ;TNF α、IL 1、IFN γ刺激后 ,人原代培养肝细胞ICAM 1表达均明显增强 ,其表达水平与细胞因子刺激之间存在一定的剂量和时间依赖关系 ;地塞米松能部分抑制TNF α、IL 1、IFN γ所诱导的人原代培养肝细胞ICAM 1增强表达 ,其抑制率分别为 (4 0 .3± 5 .9) %、(3 8.1± 4.8) %和 (3 7.6± 6.7) %。结论 IFN γ、TNF α和IL 1能诱导人原代培养肝细胞ICAM 1增强表达 ,而地塞米松能抑制其表达。
Objective To investigate the effects of IFNγ, TNFα, IL-1 and dexamethasone on the expression of intercellular adhesion molecule-1 (ICAM 1) in human primary cultured hepatocytes. Methods The human primary cultured hepatocytes were induced to express ICAM-1 by IFNγ, TNFα and IL-1. The expression of ICAM-1 was detected by cell ELISA. Dexamethasone was added before stimulation with cytokines. Effect of ICAM 1 expression in human hepatocytes. Results The expression of ICAM-1 in primary cultured human hepatocytes was very low. The expression of ICAM-1 in human primary cultured hepatocytes was significantly increased after stimulated by TNFα, IL-1 and IFNγ. The expression level of ICAM-1 was significantly higher than that stimulated by cytokines Dexamethasone could partially inhibit the expression of ICAM 1 induced by TNFα, IL-1 and IFNγ in human primary cultured hepatocytes, and the inhibitory rates were (40.3 ± 5) .9)%, (3 8.1 ± 4.8)% and (3 7.6 ± 6.7)%, respectively. Conclusion IFNγ, TNFα and IL-1 can induce ICAM-1 expression in human primary cultured hepatocytes, while dexamethasone can inhibit its expression.