目的:制备塞来昔布凝胶并建立测定塞来昔布凝胶剂含量的方法。方法:以卡波姆940为凝胶基质制备塞采昔布凝胶剂;采用高效液相色谱法测定主药含量。结果:本法制得的凝胶均匀细腻,易涂布;塞来昔布在2.05～205.20μg·ml~(-1)浓度范围内线性关系良好(r=0.999 9),平均回收率为100.01%,RSD=0.18%。结论:该方法操作简便,准确可靠。 Objective: To prepare celecoxib gel and establish a method for the determination of celecoxib gel content. METHODS: Celecoxib gels were prepared using carbomer 940 as the gel matrix. The main drug content was determined by high performance liquid chromatography. Results: The gel prepared by this method was fine and easy to coat. The calibration curve of celecoxib was linear (r = 0.999 9) in the range of 2.05 ~ 205.20μg · ml ~ (-1) with the average recovery of 100.01% , RSD = 0.18%. Conclusion: The method is simple, accurate and reliable.