采用HPLC法 ,对 18种杨属植物树皮中酚甙类成分含量进行了测定。所建立的方法 [采用Shim packODS柱 ,以甲醇 (A)和水 四氢呋喃 (99∶1) (B)为流动相 ]确实可靠 ,具良好的线性关系 ,精密度RSD<2 % ,加样回收率salireposide为 99.2 % ,RSD =1.77% ,davidianin为 98.6 % ,RSD =3.0 1% ,grandidentatin为 10 2 .1% ,RSD =0 .91%。本法可用于杨属植物的含量测定。salicin为杨属植物的共有成分 ,且含量较高 ,种间差异明显 The contents of phenolphthalein in the barks of 18 species of poplars were determined by HPLC. The established method [using Shim pack ODS column with methanol (A) and water tetrahydrofuran (99:1) (B) as mobile phase] is reliable, with good linearity, precision RSD < 2%, sample recovery Salireposide was 99.2%, RSD = 1.77%, davidianin was 98.6 %, RSD = 3.0 1%, grandidentatin was 102.1%, and RSD = 0.91%. This method can be used for the determination of poplar plants. Salicin is a common component of Populus, and its content is relatively high, with significant differences between species.