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在日本虽然自动分析器日渐普及,但使用赖特曼—弗兰克尔(R—F)法测定转氨酶的依然占其大半,且已标准化。但R—F 法还存在一些问题,尚未得到解决。本文介绍了一种利用丙酮酸氧化酶(POP)测定血清转氨酶的方法。其原理是利用谷丙转氨酶(GPT)反应生成丙酮酸,在二价磷酸根离子、溶解状态的氧和黄素腺嘌呤二核苷酸(FAD)、硫氨素焦磷酸(TPP),镁离子存在下,由于POP 的作用而生成的过氧化氢,后者和4—氨基安替比林、N,N—二甲基苯胺、过氧化物酶(POD)氧化缩合,在555nm 波长测定吸光度变化。谷草转氨酶(GOT)测定,因其反应生成草酰乙酸,在草酰乙酸脱羧酶(OAC)作用下也产生丙酮酸,其余步骤同谷丙转氨酶。
Although automatic analyzers are gaining popularity in Japan, the determination of aminotransferases by Reverent-Frankel (R-F) is still predominant and standardized. However, there are still some problems in the R-F method that have not yet been solved. This article describes a pyruvate oxidase (POP) method for the determination of serum aminotransferases. The principle is the use of alanine aminotransferase (GPT) reaction pyruvate, in the divalent phosphate ions, dissolved oxygen and flavin adenine dinucleotide (FAD), thiamin pyrophosphate (TPP), magnesium ions In the presence of hydrogen peroxide generated by the action of POP, the latter is oxidatively condensed with 4-aminoantipyrine, N, N-dimethylaniline, and peroxidase (POD) to measure the change in absorbance at a wavelength of 555 nm . Aspartate aminotransferase (GOT) assay, which generates oxaloacetate as a result of its reaction, also produces pyruvate under the action of oxaloacetate decarboxylase (OAC) and the remaining steps are the same as alanine aminotransferase.