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目的探讨钙囊素(S100A11)过表达对人胰腺癌细胞PANC-1增殖的影响。方法构建S100A11过表达载体质粒pcDNA3.1-S100A11,并转染人胰腺癌细胞PANC-1细胞。Western blot检测转染效果;CCK-8检测转染后PANC-1细胞增殖活力;流式细胞术检测PANC-1细胞的凋亡和细胞周期的变化。结果 S100A11蛋白表达量与pcDNA3.1-S100A11的转染量成正相关。过表达S100A11基因可明显提高PANC-1细胞增殖速度,降低凋亡细胞比例,并使PANC-1细胞S期细胞比例增加,G1期细胞减少(P<0.05)。结论S100A11过表达能调控PANC-1细胞周期,并促进PANC-1细胞的增殖。
Objective To investigate the effect of overexpression of calcium allantoin (S100A11) on the proliferation of human pancreatic cancer cell line PANC-1. Methods The S100A11 overexpression vector pcDNA3.1-S100A11 was constructed and transfected into human pancreatic cancer cell line PANC-1. The transfection efficiency was detected by Western blot. The proliferation activity of PANC-1 cells was detected by CCK-8. The apoptosis and cell cycle of PANC-1 cells were detected by flow cytometry. Results The expression of S100A11 protein was positively correlated with the transfection efficiency of pcDNA3.1-S100A11. Overexpression of S100A11 gene could significantly increase the proliferation rate of PANC-1 cells, decrease the proportion of apoptotic cells, increase the proportion of S phase cells in PANC-1 cells and decrease the cells in G1 phase (P <0.05). Conclusion S100A11 overexpression can regulate the cell cycle of PANC-1 cells and promote the proliferation of PANC-1 cells.