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运用人工授精方法,进行海南沼虾(♀)×日本沼虾(♂)杂交试验,获得了可育的F1代杂种,并对F1代及其双亲进行了RAPD分析。共进行6批杂交试验,4批抱卵,3批获得杂交虾,成活率分别为30%,48%,64%。形态上,F1代兼有双亲特征,但偏向父本。RAPD扩增结果显示,海南沼虾和日本沼虾的扩增带型存在显著的差异,F1杂种兼有双亲的特征带,父、母本之间的遗传距离为0.785 6,父本与F1的遗传距离为0.410 9,母本与F1的遗传距离为0.423 8。从分子水平确认了日本沼虾(♀)和海南沼虾(♂)种间杂交的成功,并为进一步寻找经济性状相关分子标记提供了条件。
Using artificial insemination method, the crossbreeding experiment of M. marxianus × P. japonicus (♂) was carried out to obtain fertile F1 hybrids, and the F1 generation and its parents were analyzed by RAPD. A total of six batches of hybridization experiments, four batches of eggs, three batches of hybrid shrimp, the survival rates were 30%, 48%, 64%. Morphology, F1 generation both parents characteristics, but biased towards the male parent. The results of RAPD amplification showed that there was a significant difference in amplification bands between Macrobrachium nipponense and Macrobrachium nipponense. F1 hybrid had the characteristic bands of parents, the genetic distance between parent and female was 0.785 6, The genetic distance was 0.410 9 and the genetic distance between female parent and F1 was 0.423 8. The success of interspecific hybridization between Macrobrachium nipponense (♀) and Macrobrachium nipponense (♂) was confirmed at the molecular level, which provided conditions for further searching for molecular markers related to economic traits.