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目的 肠三叶因子(ITF)对消化道粘膜上皮细胞的损伤修复具有重要作用。本实验通过研究ITF 对新生大鼠坏死性小肠结肠炎(NEC)模型肠组织病理学改变,环氧合酶 2(COX 2)表达及前列腺素E2(PGE2)、血 栓素B2(TXB2)生成的影响,以探讨ITF对NEC是否有治疗作用。方法 40只新生1日龄Wistar大鼠随机分为5 组,每组8只。A组为正常对照组。B和C组大鼠为NEC模型鼠,分别予以0.5mL生理盐水腹腔或0.2mL皮下注 射。D和E组大鼠亦为NEC模型鼠,分别予以0.5mLITF(0.5mg)腹腔或0.2mL(0.2mg)皮下注射。连续3天 新生大鼠予以缺氧-复氧处理制成NEC模型。第4天处死所有大鼠,取肠组织检查组织病理学改变,COX 2表达 和PGE2与TXB2的生成。结果 A组的肠组织病理学未见异常,病理评分为0分。与相应的NEC组(B和C组) 比较,ITF治疗后(D和E组)NEC导致的组织病理学改变明显减轻(P<0.01)。B和C组的病理学评分为1～4 分,而D和E组评分为0～2分。与A组比较,B和C组PGE2与TXB2浓度显著增高,但ITF治疗后(D和E组)显 著下降,与A组无明显差异。免疫组化结果显示B和C组的COX 2表达显著高于A,D,E组(P<0.05)。D和E 组弱表达COX 2,其强度高于A组,但显著低于B和C组。结论 ITF通过抑制COX 2的表达,减少了PGE2和 TXB2含量,减轻肠组织炎?
Purpose Intestinal trefoil factor (ITF) plays an important role in the repair and repair of gastrointestinal mucosal epithelial cells. The present study was designed to investigate the effects of ITF on intestinal histopathology, cyclooxygenase 2 (COX 2) expression, prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) production in neonatal rats with necrotizing enterocolitis (NEC) To investigate whether ITF has a therapeutic effect on NEC. Methods 40 newborn Wistar rats of 1 day old were randomly divided into 5 groups with 8 rats in each group. Group A is normal control group. Rats in groups B and C were NEC model rats, which were injected intraperitoneally with 0.5 mL of saline or subcutaneously with 0.2 mL respectively. Rats in groups D and E were also NEC model rats, which were injected intraperitoneally with 0.5 mL of ITF (0.5 mg) or subcutaneously with 0.2 mL (0.2 mg) respectively. Neonatal rats were hypoxic-reoxygenated for 3 days to make NEC model. On day 4, all rats were sacrificed, histopathological changes of intestinal tissue were examined, COX 2 expression and generation of PGE 2 and TXB2 were examined. Results A group of intestinal histopathology no abnormalities, pathological score was 0 points. Compared with the corresponding NEC group (B and C groups), the histopathological changes induced by NEC in the ITF group (D and E groups) were significantly reduced (P <0.01). The pathological scores of group B and C were 1 to 4, while those of group D and E were 0 to 2. Compared with group A, the concentrations of PGE2 and TXB2 in group B and group C were significantly increased, but the levels of PGE2 and TXB2 in group B and C were significantly decreased after treatment with ITF (group D and E). The results of immunohistochemistry showed that the expression of COX 2 in B and C groups was significantly higher than that in A, D and E groups (P <0.05). In group D and E, the expression of COX 2 was weaker than that in group A, but significantly lower than those in group B and C. Conclusion ITF can inhibit the expression of COX 2, reduce the contents of PGE2 and TXB2 and relieve intestinal inflammation.