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以聚苯乙烯纳米球为研究对象,研究基质硬度对二维(2D)和三维(3D)培养的乳腺癌细胞摄取行为的影响。通过光催化聚合反应法制备硬度可调的聚丙烯酰胺凝胶,并在凝胶表面共价修饰胶原以支持细胞黏附,构建2D细胞培养模型;选择Ⅰ型鼠尾胶原构建3D细胞培养模型;分别接种人乳腺癌细胞MCF-7于聚丙烯酰胺凝胶表面或混悬于胶原凝胶中进行3D细胞培养;20 nm和50 nm聚苯乙烯纳米球悬液与2D或3D培养的细胞共孵育,采用激光共聚焦显微镜对细胞形态和纳米球摄取效率进行定性和定量考察。本研究发现在2D和3D培养条件下,基质硬度均参与调控肿瘤细胞肌动蛋白骨架的重构并间接影响不同粒径大小的纳米制剂的摄取行为。
Polystyrene nanospheres were used to investigate the effects of matrix hardness on uptake by breast cancer cells in two-dimensional (2D) and three-dimensional (3D) cultures. The polyacrylamide gel with adjustable hardness was prepared by photocatalytic polymerization and the collagen was covalently modified on the surface of the gel to support cell adhesion. The 2D cell culture model was constructed. The type Ⅰ mouse tail collagen was used to construct 3D cell culture model. Inoculation of human breast cancer cells MCF-7 on polyacrylamide gel surface or suspended in collagen gel for 3D cell culture; 20 nm and 50 nm polystyrene nanosphere suspension cells incubated with 2D or 3D, Laser confocal microscopy was used to qualitatively and quantitatively investigate cell morphology and nanosphere uptake efficiency. The present study found that in both 2D and 3D culture conditions, matrix hardness is involved in the regulation of tumor actin cytoskeleton remodeling and indirectly affects the uptake behavior of different size nanoparticle preparations.