建立补骨脂药材快速、简便的含量测定方法,考察不同产地药材中补骨脂素和异补骨脂素的含量差异。采用单因素和正交试验设计法考察最佳提取条件,采用薄层-紫外法分离测定补骨脂素和异补骨脂素。结果不同产地补骨脂中补骨脂素含量为3.357～5.768mg/g,异补骨脂素含量为2.235～5.354mg/g,广西补骨脂药材中补骨脂素和异补骨脂素含量较高。用本法测定,操作简便、准确性高、重复性好。 To establish a rapid and simple method for the determination of psoralen, and to investigate the content differences of psoralen and isopsoralen in medicinal materials from different areas. The optimal extraction conditions were investigated by single factor and orthogonal experimental design. Psoralen and isopsoralen were separated and determined by TLC-UV. Results The content of psoralen in different regions of Psoralen was 3.357-5.758mg / g, the content of isopsoralen was 2.235-5.354mg / g, the content of psoralen and isopsoralen in Guangxi psoralen High content. Measured by this method, easy to operate, high accuracy, good repeatability.