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目的:研究中药复方有效部位(TCM-ES)的体外抗流感病毒FM1株(IFV-FM1)的作用。方法:采用微量细胞病变抑制法、中性红染色法、空斑抑制法,从TCM-ES的最大无毒浓度开始,按Reed-Muench法计算药物对FM1感染狗肾传代细胞(MDCK)的半数有效浓度(EC50),最小有效浓度(MTC),治疗指数(TI)。进行空斑计数(PFU),计算其半数抑制浓度(IC50)。结果:微量细胞病变抑制法表明,TCM-ES抗FM1的EC50为(300±18.3)mg/L,MTC为(75±6.8)mg/L,TI为(7.1±0.7);而利巴韦林的3项指标分别为(52.3±10.1)mg/L、(25±4.1)mg/L和(20.8±5.1)。中性红染色法显示,两药物的IC50分别为(285.0±19.2)mg/L和(45.3±4.9)mg/L,TI分别为(7.2±0.6)和(21.2±3.1)。两种方法测定的TCM-ES和利巴韦林的TI值无统计学差异(P>0.05)。通过PFU计数,TCM-ES的半数抑制浓度(IC50)为300mg/L,利巴韦林的IC50为50mg/L,与前述结果亦无统计学差异(P>0.05)。结论:TCM-ES体外具有一定的抗流感病毒作用,可作用于细胞内病毒,但其效果略差于利巴韦林,具有进一步研究和开发的价值。
Objective: To study the effect of in vitro anti-influenza virus FM1 strain (IFV-FM1) on TCM-ES. Methods: Using the method of Reed-Muench, the half-number of MDCK in FM1-infected canine kidney cells (MDCK) was calculated by MTT assay, neutral red staining and plaque inhibition assay. Effective concentration (EC50), minimum effective concentration (MTC), therapeutic index (TI). Plaque counts (PFU) were taken and the half-inhibitory concentration (IC50) calculated. Results: The results showed that the EC50 of TCM-ES against FM1 was (300 ± 18.3) mg / L, the MTC was (75 ± 6.8) mg / L and the TI was (7.1 ± 0.7) The three indicators were (52.3 ± 10.1) mg / L, (25 ± 4.1) mg / L and (20.8 ± 5.1) respectively. The neutral red staining showed that the IC50 of the two drugs were (285.0 ± 19.2) mg / L and (45.3 ± 4.9) mg / L, respectively, and were respectively 7.2 ± 0.6 and 21.2 ± 3.1 for TI. There was no significant difference in TI values between TCM-ES and ribavirin measured by the two methods (P> 0.05). The median inhibitory concentration (IC50) of TCM-ES was 300mg / L and the IC50 of ribavirin was 50mg / L by PFU counting. There was no significant difference with the above results (P> 0.05). Conclusion: TCM-ES has certain anti-influenza virus effect in vitro and can act on intracellular virus, but its effect is slightly worse than ribavirin, which has the value of further research and development.