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冷锻炼和ＡＢＡ处理提高了水稻幼苗叶绿体ＳＯＤ和ＧＲ活性及叶片抗氧化剂ＡｓＡ和ＧＳＨ的含量，降低了膜电解质泄漏，增强了幼苗的抗冷性．等电聚焦电泳分析表明，冷锻炼和ＡＢＡ处理苗叶绿体ＳＯＤ三条同工酶带和ＧＲ１、２、３和６同工酶带都有不同程度的增强．低温胁迫后，处理和未处理首的ＳＯＤ、ＧＲ活性和ＡＳＡ、ＧＳＨ含量均有所下降．但处理苗的水平仍维持在未处理苗之上．亚胺环已酮可抑制因冷锻炼和ＡＢＡ诱导增加的ＳＯＤ和ＧＲ活性，并使叶片电解质泄漏增大．本试验结果表明冷锻炼或ＡＢＡ诱导水稻幼苗抗冷性提高时，对防御活性氧的保护系统有类似的影响。
Cold exercising and ABA treatments increased SOD and GR activities in chloroplasts and the content of AsA and GSH in leaves of rice seedlings, decreased membrane electrolyte leakage and enhanced chilling tolerance of seedlings. Isoelectric focusing electrophoresis analysis showed that three isozymes of chloroplast and two isozymes of GR1, 2, 3 and 6 were enhanced to some extent by cold acclimation and ABA treatments. After low temperature stress, the activity of SOD, GR and the contents of ASA and GSH decreased in the treated and untreated ones. However, the level of treated seedlings remained above that of untreated seedlings. Iminocyclohexanone inhibited SOD and GR activities induced by cold acclimation and ABA induction, and increased leaf electrolyte leakage. The results of this experiment show that cold-stress or ABA-induced increase of chilling tolerance in rice seedlings has a similar effect on the protective system against reactive oxygen species.