70年代已肯定 E 花结形成是 T 淋巴细胞的独特标志,而活性 E 花结(Ea)是总 T细胞中具有活性功能的一个亚群。近年来许多报道指出,酸性α—醋酸綦酯酶(ANAE)活性与 E 花结形成能力有相关性,并认为在严格控制实验条件下,ANAE 阳性细胞的阳性率与 Ea 形成率十分接近,所以ANAE 阳性细胞基本可以代表正常成熟的静止的 T 细胞,而且将 ANAE 活性产物视为T 细胞的内标记,用做细胞免疫测定法之 The 1970s have affirmed that E-nodal formation is a unique hallmark of T-lymphocytes, whereas E-reactive (Ea) is a subset of all T-cells that have an active function. In recent years, many reports point out that the activity of acid α-acetate 綦 esterase (ANAE) is related to the formation of E-rosette and that the positive rate of ANAE-positive cells is very close to that of Ea under the strict control of experimental conditions ANAE positive cells can basically represent normal mature resting T cells, and the ANAE active product is regarded as an internal marker of T cells and used as a cellular immunoassay