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目的:观察葛根素对新发现的小分子活性肽Apelin在大鼠心肌缺血/再灌注损伤中表达的影响。方法:将SD大鼠60只,随机分成4组:缺血/再灌注(IR)组即缺血20min后再灌注180min;葛根素+缺血/再灌注(IP)组;假手术(SH)组和正常对照(NC)组。体表心电图标Ⅱ导联全程记录,进行心律失常得分评估;用放免法测定各组大鼠血浆中Apelin-36蛋白含量变化;RT-PCR方法观察心肌Apelin mRNA表达。结果:(1)葛根素IP组心律失常得分仅为IR组的69.9%(P<0.01)。(2)血浆Apelin-36浓度:SH组、IR组、IP组分别是NC组93.4%(P>0.05)、51.5%(P<0.01)和73.5%(P<0.01)。IP组比IR组含量高,两者有统计学差异(P<0.01)。(3)心肌Apelin mRNA的表达:IR组明显降低,仅为NC组的40.2%(P<0.01);IP组为NC组的68.1%(P<0.01);IP组比IR组高(P<0.05)。结论:药物葛根素处理后能使大鼠血浆Apelin-36含量及心肌中ApelinmRNA表达升高。提示葛根素能影响小分子活性肽Apelin在大鼠心肌缺血/再灌注损伤过程中的表达。
Objective: To observe the effect of puerarin on the expression of newly discovered small molecule active peptide Apelin in myocardial ischemia/reperfusion injury in rats. Methods: Sixty Sprague-Dawley rats were randomly divided into 4 groups: ischemia/reperfusion (IR) group: ischemia 20 min and then reperfusion 180 min; puerarin + ischemia/reperfusion (IP) group; sham operation (SH) Group and normal control (NC) groups. Body surface ECG II leads were recorded throughout the course and arrhythmia scores were assessed; plasma Apelin-36 protein levels in rats were determined by radioimmunoassay; and myocardial Apelin mRNA expression was observed by RT-PCR. Results: (1) The score of arrhythmia in puerarin IP group was only 69.9% in IR group (P<0.01). (2) Plasma Apelin-36 concentrations: 93.4% (P>0.05), 51.5% (P<0.01), and 73.5% (P<0.01) of plasma in the SH group, IR group, and IP group, respectively. The IP group had higher levels than the IR group. There was a statistical difference between the two groups (P<0.01). (3) The expression of Apelin mRNA in myocardium: IR group was significantly decreased, only 40.2% (P<0.01) in NC group; IP group was 68.1% in NC group (P<0.01); IP group was higher than IR group (P< 0.05). CONCLUSION: The puerarin treatment can increase the plasma Apelin-36 content and the expression of Apelin mRNA in myocardium. It was suggested that puerarin could affect the expression of small molecule active peptide Apelin during myocardial ischemia/reperfusion injury in rats.