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目的研究石家庄市食源性蜡样芽胞杆菌毒力基因的分布及毒力活性,了解蜡样芽胞杆菌的潜在威胁。方法采用PCR方法,对食品风险监测中分离到的131株蜡样芽胞杆菌进行肠毒素、呕吐毒素9种毒力基因扩增检测,用血平板检测的方法分析蜡样芽胞杆菌的毒力。结果毒力基因携带率较高,至少携带一个毒力基因的菌株达到检出菌总数的99.2%(130/131),溶血素BL基因(hbl ACD)和肠毒素FM基因(ent FM)是石家庄市食源性蜡样芽胞杆菌的主要毒力基因;检出的蜡样芽胞杆菌均产生溶血素BL,检出率为100%。结论腹泻型肠毒素在食品中的分布比较广泛,检出的蜡样芽胞杆菌均具有溶血素,对进食者存在潜在的危险性,今后应加强监控蜡样芽胞杆菌的污染,预防和控制蜡样芽胞杆菌食源性疾病的发生。
Objective To study the distribution and virulence of foodborne Bacillus cereus in Shijiazhuang and to understand the potential threat of Bacillus cereus. Methods Ninety-two virulence genes of enterobacterial and mycotoxin isolated from 131 strains of Bacillus cereus isolated from food risk monitoring were detected by polymerase chain reaction (PCR). The virulence of B. aeruginosa was assayed by blood plate test. Results The virulence gene carrying rate was higher, at least 99.2% (130/131) of the total number of strains carrying virulence genes were detected. The hemagglutinin BL gene (hbl ACD) and enterotoxin FM gene (ent FM) The main virulence genes of the food-borne Bacillus cereus in the city; Bacillus cereus all detected hemolysin BL, the detection rate was 100%. Conclusions Diarrhea enterotoxins are widely distributed in food. The detected Bacillus cereus all have hemolysin, which is potentially dangerous to the eater. In the future, it is necessary to monitor the contamination of Bacillus cereus and prevent and control wax samples Bacillus food-borne diseases.