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在pH 4.5的HAc-NaAc缓冲液中,茜素红(ARS)-镧与牛血清白蛋白(BSA)形成三元离子缔合物,导致共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)显著增强,光谱最大散射波长分别位于373 nm、620 nm和310 nm。体系的光散射强度与BSA浓度在一定范围内呈线性增强,线性范围分别是RRS在0.034～13.40μg/mL、SOS在0.201～13.40μg/mL和FDS在0.201～10.05μg/mL范围内,对于BSA的检出限分别为0.010μg/mL(RRS法)0、.052μg/mL(SOS法)和0.089μg/mL(FDS法),据此建立了灵敏的测定BSA的共振线性(RRS)和共振非线性光散射(RNLS)分析法。以RRS法考察了茜素红-镧与白蛋白形成三元离子缔合物的适宜条件、影响因素等,此法可用于血清、卵清和尿样中蛋白含量的测定。
Alizarin red (ARS) -lanthanum forms a ternary ion association with bovine serum albumin (BSA) in pH 4.5 HAc-NaAc buffer, resulting in Resonance Rayleigh Scattering (RRS), Secondary Scattering (SOS) And frequency doubling scattering (FDS) were significantly enhanced, the maximum spectral scattering wavelength located at 373 nm, 620 nm and 310 nm. The linear range of RRS was 0.034 ~ 13.40μg / mL, SOS was 0.201 ~ 13.40μg / mL and FDS was in the range of 0.201 ~ 10.05μg / mL, The detection limits of BSA were 0.010μg / mL (RRS method), 0.052μg / mL (SOS method) and 0.089μg / mL (FDS method) Resonance Nonlinear Light Scattering (RNLS) Analysis. The RRS method was used to investigate the suitable conditions, influencing factors and the like for the formation of ternary ion association of alizarin red-lanthanum with albumin. This method can be used for the determination of protein content in serum, egg white and urine samples.